In Vitro Screening

In Vitro Screening Services

Accelerating the journey from hit identification to lead optimization requires a high-throughput in vitro screening platform that can rapidly and cost-effectively characterize your most promising compounds. BioLegacy’s services provide this critical advantage, offering a comprehensive suite of physiologically relevant cell-based assays to elucidate mechanism of action, confirm target engagement, and define your therapeutic’s activity. With advanced analytical capabilities, we deliver robust, decision-driving data to confidently prioritize your development pipeline.

  • Diverse Cell-Based Assays including cytotoxicity, cell proliferation, apoptosis, and cell signaling to determine compound efficacy and MOA
  • Immuno-Oncology & Immunology Expertise Evaluate immunomodulatory agents, including T-cell and B-cell function, macrophage activity, ADCC/CDC, and cytokine release assays.
  • Custom Assay Development BioLegacy can design, optimize, and validate novel cell-based assays for emerging targets and unique therapeutic modalities.
  • Extensive Cell Line Library Access to over 350 cancer cell lines, primary cells, and drug-resistant lines to ensure the most clinically relevant model is used.
  • Advanced Bioanalytical Readouts including flow cytometry (FACS), ELISA, and multiplex platforms for in-depth analysis.
  • Antibody-Drug Conjugate (ADC) Evaluation Specialized in vitro assays to assess the potency, specificity, and safety of ADC candidates.
  • Biomarker & Pharmacodynamic Analysis Identification, validation, and quantification of key biomarkers.

Cell-Based Functional & Phenotypic Assays

To understand a compound’s therapeutic potential, it is essential to first characterize its activity in a physiologically relevant cellular context. BioLegacy’s in vitro screening platform provides a comprehensive suite of robust, cell-based functional assays designed to deliver clear, quantitative data on your compound’s efficacy and mechanism of action. Leveraging our extensive library of over 350 cancer cell lines, primary cells, and drug-resistant variants, we provide the critical data needed to confidently de-risk your program and select optimal leads.

  • Angiogenesis
  • Antibody production and characterization
  • Apoptosis
  • Calcium Flux
  • Cell adhesion
  • Cell cycle analysis
  • Cell culture and expansion of various primary and cancer cells
  • Cell differential count of lung infiltrating cells in asthma studies by cell cytospin staining and microscopic evaluation
  • Cell invasion
  • Cell migration
  • Cell morphology
  • Cell proliferation
  • Cell viability (cytotoxicity)
  • Cellular uptake of FITC-labeled drug
  • Chemotaxis
  • Custom in vitro assays
  • Dendritic cell vaccine for cancer models treatment
  • Drug degradation ex vivo in whole blood
  • Endotoxin measurement (LAL assay)
  • Ex vivo antibody production
  • Heparin compatibility drug evaluation
  • Hypoxic studies
  • In Vitro hair growth
  • Intracellular pH change
  • Mast cell isolation and histamine release in vitro
  • Mechanism of action, intracellular signaling pathway identification
  • Oxygen/glucose deprivation: evaluation of anti-apoptotic laser treatment in rat CNS cell culture
  • Receptor binding
  • Soft Agar Colony Formation Assay
  • Stem cell batch release
  • Stem cell viability
  • Primary Cell Function Assays Open Details

Holistic Toxicology Study Support

Demonstrating a clear link between drug exposure, target engagement, and biological response is essential for de-risking your therapeutic and building a compelling case for advancing a candidate. BioLegacy provides comprehensive services for the discovery, validation, and quantitative analysis of pharmacodynamic and disease-related biomarkers. Combined with our extensive bioanalytical capabilities, our in vitro biomarker services confident, data-driven program decisions.

  • Biomarker identification
  • Biomarker validation
  • Flow cytometry (cell surface and intracellular staining)
  • Western blot
  • Zymogels for protease activity
  • ELISA (development, validation and use of commercial kits)
  • Multiplex technology for cytokines
  • Pharmacodynamic studies: quantification of drug target inhibition
  • Factor VII A measurement in dogs and mice
  • HSV2 and Clamydia measurement by Q-RTPCR
  • Nitrite/nitrate measurements
  • Butyrylcholinesterase and Carboxylesterase-1 and -2 by Western Blot
  • Beta amyloid measurement by ELISA
  • DsDNA IgG measurements by ELISA
  • Hydroxyproline measurement to evaluate anti-liver fibrosis drug
  • Much more - ask us!

Immunology & Immuno-Oncology Assays

BioLegacy’s sophisticated portfolio of in vitro assays utilizing primary human and animal immune cells rapidly and resolutely characterize the activity of novel immunomodulators and biologics. From T-cell activation to antibody-dependent cellular cytotoxicity, our platforms are engineered to provide definitive insights into your compound’s immunological efficacy and mechanism of action.

  • Primary Immune Cell Assays Functional evaluation of T-cells, B-cells, NK cells, macrophages, and dendritic cells.
  • Immuno-Oncology Co-Culture Models to assess immune cell-mediated tumor killing and checkpoint inhibition.
  • ADCC & CDC Assays to evaluate the effector function of therapeutic antibodies.
  • Cytokine Release & Profiling Multiplex analysis to measure immunomodulatory effects and assess safety risks.
  • Mixed Lymphocyte Reaction (MLR) assay to evaluate T-cell proliferation and alloreactivity.

When your program demands a unique methodological approach, our team of expert scientists provides comprehensive custom assay development services, from initial concept to a fully validated, screen-ready assay. With our in-house bioanalytical and clinical pathology platforms at your disposal, we ensure that every study is supported by the robust, quantitative biomarker and safety data needed for clear interpretation.

  • De Novo Assay Development and validation for novel targets and pathways.
  • Biomarker Identification & Validation for pharmacodynamic and disease-related biomarkers.
  • Flow Cytometry & Western Blot Multi-platform support for target engagement and protein expression analysis.
  • ELISA & Multiplex Platforms Sensitive, high-throughput quantification of key protein biomarkers.
  • GLP-Compliant Clinical Pathology for analyzing samples from IND-enabling in vivo studies.

Confidently Optimize Your Pipeline

Trust BioLegacy’s in vitro screening for thorough hit-to-lead optimization.

  • Rapid, high-throughput lead optimization
  • Hundreds of cell-based assays
  • Advanced bioanalytical capabilities
  • Biomarker discovery & validation
  • Custom assay development
Let’s advance your program! Contact us today.